Development of S. lycopersicum var. MoneyMaker (NR0080) × S. pimpinellifolium TO-937-15 (NR0937) IL population
A single F1 plant from the cross between SL and SP was backcrossed with the recurrent parent generating 100 BC1 plants which were backcrossed with MM. A total of 16 BC2 plants (with the entire genome represented at least twice with overlapping donor segments and with lowest possible proportion of the donor genome) were selected and backcrossed to obtain 248 BC3 plants. A total of 76 BC3 plants were selected and divided into two groups depending on the number of introgressions: an elite group of 41 plants (each carrying three or fewer introgressions) and a backup set with 35 plants (each carrying three or more introgressions).The selected BC3 plants were genotyped and further backcrossed and selfed to obtain BC4 and BC3S1 families, respectively. Depending on the number of introgressions, BC4 or BC3S1 seedlings were screened with the appropriate SNPs by HRM in order to select plants with single (target) introgressions